If you run a realtime PCR on your cDNA samples and you get t

If you run a real-time PCR on your cDNA samples and you get the following plot, what does it mean and what are the possible causes?

Solution

The RT PCR image indicates no amplification has taken place. Either the cDNA is degraded or the probe is not working optimally. Most common causes are,

Long term storage below -20 and multiple freeze thaw can weaken the bond between the probe and conjugated fluorophore.