Describe what would be seen under a brightfield vs darkfield

Describe what would be seen under a brightfield vs darkfield microscope viewing sphagnum moss as the specimen. What are some of the characteristics and descriptions of the sphagnum moss.

Solution

Bright field microscopy allows low contrast of the organism. Presence of chloroplast and cell outline can be visualised for Sphagnum, with the help of brightfield microscopy.

In dark field microscopy, the surrounding field around the specimen appears dark. Broad cell outline of Sphagnum can be observed.

Characteristics of Sphagnum moss-

1. The plant grows in clumps to form a cushion floating over water. No vascular tissue is present.

2. Leaves are small, simple and form hair like tufts. Leaves have dead cells alternating with living cells.

3. Roots are absent, instead rhizoids are present for anchorage.