Homework SourseIn light of the high throughput and economy of the 2nd Gen s
In light of the high throughput and economy of the 2nd Gen sequencing technologies, when/where might you still rely on Sanger dideoxy sequencing in your experiments?
In light of the high throughput and economy of the 2nd Gen sequencing technologies, when/where might you still rely on Sanger dideoxy sequencing in your experiments?
Solution
ANS:
Sanger Method:
Sanger Method is a Form of DNA Synthesis. In this method the new of DNA strand is enzymatically synthesized by existing DNA strand. In this the old DNA strand was sequenced and acts like a template.
Next Generation sequencing (NGS):
In this the DNA is sequenced by collective technologies, it is developed by:
Roche 454 sequencing
Illumina (solexa) sequencing
Solid sequencing
2nd Generation sequencing technologies used in Sanger di de oxy sequencing experiment:
Where you need precision or in a difficult to access gene or when you only need to sequence a small amount of a genome.
