how to find Mimic pglo expression in eukaryotic cells give d

how to find Mimic pglo expression in eukaryotic cells? give details

Solution

In eukaryotic cells.pglo expression is used to study gene regulation and genetic selection.This plasmid contains arabinose promoter and gene for Green Fluoresecent Protein, the expression of which supports the expression of pglo in eukaryotic cells.The expression of GFP takes place in the cell when arabinose is included in the growth medium.For selection of cells that are transformed with pglo. antibiotic resistance mechanism is used.The transformed cells will appear white in media containing Ampicillin and no arabinose while it will appear green in media containing ampicillin and arabinose.