A research group discovered a new enzyme happyase that catal

A research group discovered a new enzyme happyase that catalyzes chemical reaction
Sad_student <-> Happy_student

Researchers characterized this enzyme and found that at [Etotal]=10nM, this enzyme has a Vmax at 2.5 microM/s. it was also experimentally measured that at [Sad_student]=0.05 mM this enzyme has V0=500 nM/s. what are the
kcat and Km of the happyase enzyme? Include units of measure.

STUDENT\'S NAME: Question points, 1 point or each correct answer0: A research group discovered a new enzyme happyase thatcatalyzes chemical reaction SAD STUDENT HAPPY STUDENT esearchers characterized this enzyme and found that at 0 nM this enzyme has V 2SuMMs. It was also ex perimentally measured that a SAD STUDENT 0.06 mM this enzyme has V 500 nM/s. What are the kon and Km of the happyase enzyme? Make sure to write down Kan\" Question 2 (1 point: Sample of DNA isolated from bacteriam X conains 17.5% of aderine bases. What percentages of gaanine, thymine, and cylosine bases would you expect find in the same sample of DNA?

Solution

Michaelis and Menten reaction is given by

Vo= Vmax[S]/Km + [S]

Km + [S] = Vmax[S]/Vo

Km = (Vmax[S]/Vo) – [S]

          2500*0.05/500 – 0.05

Km = 0.2 sec^-1

Vo =k_cat[E_t][S]/Km

k_cat = Vo Km/[E_t][S]

           500*0.2/10*0.05

k_cat = 200 Sec^-1