1 To isolate a gene a genetic engineer might need 2 Cloning

1. To isolate a gene, a genetic engineer might need:

2. Cloning vectors used in DNA technology are usually:

3. Genomic libraries usually contain:

4. Complimentary DNA is made with the help of:

5. cDNA:

6. In nucleic acid hybridization bacterial colonies with the gene of interest are identified by:

7. A DNA fingerprint is possible because our DNA contains many stretches of base sequences collectively known as:

8. In an electric field DNA migrates to which pole ___? Why ___?

9. The first stage in creating a genetic map is to:

10. To sequence DNA using the dideoxy chain-termination method, which of the following would not be used?

Solution

1. To isolate a gene, a genetic engineer might need: gene cloning using plasmid

2. Cloning vectors used in DNA technology are usually: plasmids

3. Genomic libraries usually contain: cDNA

4. Complementary DNA is made with the help of: mRNA

5. cDNA: Complementary DNA

6. In nucleic acid hybridization bacterial colonies with the gene of interest are identified by: selective media using antibiotics. Bacteria are generally transformed with antibiotic resistant genes

7. A DNA fingerprint is possible because our DNA contains many stretches of base sequences collectively known as: short tandem repeats (STRs)

8. In an electric field DNA migrates to which pole ___? Why ___?

DNA migrated to positive pole since it is negatively charged (presence of phosphodiester backbone)