The horizontal blocks below help summarize functional featur

The horizontal blocks below help summarize functional features associated with a proteins amino add sequence with the N terminal at left and the C-terminal at right. For the two proteins displayed SP Indicates a signal peptide, and GFP is green fluorescent protein. The single letters at the C-terminus of the constructs are amino acid abbreviations. Based on your understanding of protein secretion in eukaryotic cells, at the right of each protein predict all locations In the plant cell where you would expect to see fluorescence by GFP after expression in plant cells and examination for fluorescence. Now use vertical lines to divide the rectangle below into the functional domains for the protein coded by the gene you studied in lab. Label the functional domains. Provide a description of the function or activity associated with each functional domain. Summarize by describing what the collection of actrvit.es would allow the protein to do. In your summary be sure to comment on locations in the cell you would expect to find your protein while providing explanation of how the protein was targeted to those positions.

Solution

The SEKDEL sequence of signal peptide leads to retention of the protein in the endoplasmic reticulum of the cell. Hence the protein with GFP and SEKDEL will be seen on endoplasmic reticulum. Since there is no signal peptide in the first protein the protein will be located in the cytoplasm of the cell.

The vertical line can be divided as N terminal signal peptides - gene sequence - C terminal signal peptides. Where the N terminal signals for post translational modification and C terminal signals provides information of protein targeting.