Microbiology Question 1 In order to transfer an F factor mus

Microbiology Question

1) In order to transfer, an F factor must have which of the following?

-oriV and its own DNA polymerase

-oriV and tra genes

-oriT and tra genes

-oriT and its own DNA polymerase

-oriT, tra genes, and its own DNA polymerase

2)Knockout mutations can include ____ mutations and ______ mutations.

-gain of function; loss of function

-gain of function; nonsense

-frameshift; nonsense

-silent; nonsense

-silent; missense

Solution

Answer:

1). C. oriT and tra genes.

Explanation:

The most common functional segments constituting F factors are: OriT (Origin of Transfer): The sequence which marks the starting point of conjugative transfer.OriC (Origin of Replication): The sequence starting with which the plasmid-DNA will be replicated in the recipient cell. tra-region (transfer genes): Genes coding the F-Pilus and DNA transfer process.

2). -frameshift; nonsense