An org cys his met pro bacterial strain is known to be lysog

An org^+ cys^+ his^+ met pro bacterial strain is known to be lysogenic for a newly discovered phage that undergoes specialized transduction. The bacterial chromosome map is... The lysogenic strain is used as a source of the phage, and the phages are added to a bacterial strain of genotype org cys his met pro. After a brief incubation, samples of these phage-exposed bacteria are plated on five different types of minimal media (1-5), with the supplementations as indicated in the following table. A plus sign means the minimal media contained that particular supplement (indicated at the top of each column), a minus sign means the media was missing that supplement. Based on this information, tell me whether or not you\'d find colonics (growth) on each of the five media if specialized transduction occurred. OK cool. Why would those media have grown colonies?

Solution

a)Growth on each of the five media are:

1) N

2) C

3) N

4) C

5) N

N= no colonies are formed,C = colonies are formed

b)The media have grown colonies because these selected for cys+ or met+ transductants and indicate that the prophage is located in cys-met region.