PART I Directions In the DNA Haystacks below you are given

PART I ~ Directions: In the DNA “Haystacks” below you are given a normal DNA strand and it’s altered but similar DNA strand. Your first job is to analyze each pair and identify and name the type of genetic “alteration”. Next, use the mRNA codonà Amino Acid chart to decode both strands and identify exactly how the “alteration” changed or confused “genetically coded message”.
Normal “Sense” Strand  ~ ATGAAAAACAAGGTACACATCTAG
mRNA Strand~ _____________________________________________________________ Polypeptide~ _______________________________________________________________

Altered “Sense” Strand  ~ ATGAAAAACAACGTACACATCTAG
mRNA Strand~ _____________________________________________________________ Polypeptide~ _______________________________________________________________ Circle the “altered” section and identify the kind of alteration: ________________________

Normal “Sense” Strand ~ ATGAAAAACAATTGCACGTAG
mRNA Strand~ _____________________________________________________________ Polypeptide~ _______________________________________________________________

Altered “Sense” Strand ~ ATGAAAAACAATTGCACGTG
mRNA Strand~ _____________________________________________________________ Polypeptide~ _______________________________________________________________

Solution

Sense” Strand       ATGAAAAACAAGGTACACATCTAG
mRNA Strand        UACUUUUUGUUCCAUGUGUAGAUC
Polypeptide      
1 UAC UUU UUG UUC CAU GUG UAG AUC 24
1 Tyr Phe Leu Phe His Val End Ile 8

Sense” Strand       ATGAAAAACAACGTACACATCTAG
mRNA Strand        UACUUUUUGUUGCAUGUGUAGAUC
Polypeptide      
1 UAC UUU UUG UUG CAU GUG UAG AUC 24
1 Tyr Phe Leu Leu His Val End Ile 8

ATGAAAAACAA G GTACACATCTAG
ATGAAAAACAA C GTACACATCTAG

The mutation is G to C. It is Transversion type of muation