7 Induction of Heat shock proteins such as HSP70 in Drosophi

7) Induction of Heat shock proteins such as HSP70 in Drosophila requires the binding of phosphorylated__________________ to specific ________________ and is an example of __________________ regulation of _________________

8) Transcription factors can bind to

a. Promoters

b. Enhancers

c. Silencers

d. Each other

e. Repressors

f. b+c

g. a+b+c+d

9.) Which of the following is considered a Local Histone Mobilization (circle all the correct ones and explain each)

a. Ejection

b. Phosphorylation

c. Sliding

d. Methylation

e. Unwrapping

What is the name of the complex that mediates this/these activit(y/ies)

Solution

Answer:

7. Induction of Heat shock proteins such as HSP70 in Drosophila requires the binding of phosphorylated hstf to specific hse heat shock response elements and is an example of transcription regulation of RNA polymerase binding.

8. (g) a+b+c+d.

Basal transcription factors bind first to core promoter, and then RNAP binds to them. They also bind to areas outside the core promoter, usually to enhancers or silencers (distal control elements).

9. (a), (c), (d), (e)

Ejection: ATP-dependent chromatin-remodeling complexes regulate gene expression by either moving, ejecting or restructuring nucleosomes.

Sliding: Chromatin remodelers use the energy of ATP to mobilize DNA around nucleosomes. Remodeler activity can result in nucleosome sliding, eviction, or replacement of canonical histones with specific histone variants.

Methylation: Histone methylation is defined as the transfer of one, two, or three methyl groups from S-adenosyl-L-methionine to lysine or arginine residues of histone proteins by histone methyltransferases

Unwrapping: Nucleosomes are stable DNA-histone protein complexes that must be unwrapped and disassembled for genome expression, replication, and repair.

SWI/SNF complex family (SWitch/Sucrose NonFermentable) mediates these activities.