Application varies total Thermophilic fungal can thrive in

Application: varies - total Thermophilic fungal can thrive in temperatures extending up to 62degree C (143degreeF). As the only representatives of eukaryotic organisms that can grow at such extreme temperatures, the thermophilic fungi are valuable experimental systems for investigators. Although widespread in terrestrial habitats, they have remained underexplored compared to thermophilic species of bacteria. you are most interested in the molecular details of DNA replication in a newly isolated thermophilic fungus you have maintained in your laboratory. You are anxious to identify its DNA replicator sequence. You intend to use the same method utilized by researchers studying both prokaryotic and eukaryotic DNA replication to identify sequences that can serve as replicator. Describe in detail the screen you would use to identify potential DNA replicator sequences. Use pictures to illustrate if necessary, but include text to explain.

Solution

answer:

Thermophilic fungi is undoubtedly new idea in biotechnology. To gain insight into the molecular mechanism of DNA replicators it warrants sequence technology. There are several ways to determine the sequence however metagenomics and sequencing by Madam Gilbert is praised well and applicable here. Besides four bases of DNA, digitonin required as a detergent and DNA replicator specific radioactive probe are necessary for the process.