Homework SourseWhich of the two primers below will most likely have a HIGHE
Which of the two primers below will most likely have a HIGHER melting temperature (Tm) ? Choose the most accurate answer.
Primer #1: GGAATTGGTGCAGTG
Primer #2: TTACTTCAAAATAATCT
A)Primer #2 Because it is longer
B)Primer #1 Because it there are no long stretches of the same nucleotide
C)Primer #1 Because it has a lower AC content
D)Primer #2 Because it is easier to melt
E)Primer #1 Because it has higher GC content
| A)Primer #2 Because it is longer | ||
| B)Primer #1 Because it there are no long stretches of the same nucleotide | ||
| C)Primer #1 Because it has a lower AC content | ||
| D)Primer #2 Because it is easier to melt | ||
| E)Primer #1 Because it has higher GC content |
Solution
answer is E) as in primer 1 GC content is higher in which three hydrogen bonds are there which takes higher energy to form.
