Write down the results that you expect to see on each of the

Write down the results that you expect to see on each of the agar plates: Your predictions should relate to the bacterial growth characteristics and the ability of the cells to fluoresce (glow). You should also include the reasoning behind your predictions for each plate

Solution

The gene encoding green fluorescent protein (GFP) was originally isolated from Aequora victoria, a bioluminescent jellyfish. The pGLO plasmid contains both the gene for GFP as well as a gene for resistance to the antibiotic ampicillin. Once pGLO has been inserted into a bacterial cell, adding the sugar arabinose can control expression of GFP. Transformed cells will appear white under incandescent light and fluorescent green under UV light.

In a cell with no arabinose present the AraC protein bound to the DNA in wrong configuration this means the transcription is suppreseed and GFP would not be expressed and fluorescent would be absent.

Once the cell is able to take arabinose trasncription take place and the GFP is expressed and imparts its fluorescent.

where in case of plasmid containing , a plasmid should have a ampicillin resistant gene and also presence of arabinose.

The plasmid imparts grren fluorescent colour where both arabinose and ampicillin resistant gene is present.