Keeping in mind the assays done in Lab 12 a how could you de

Keeping in mind the assays done in Lab 1/2, (a) how could you design a colorimetric method to measure alpha-amylase activity? (b) What would you use as a control? (c) At what wavelength should the spectrophotometer be set? (d) Sketch a graph like Plot 1 (from this lab) to represent the amylase activity you would observe using your method. You want to know whether alpha-amylase is more active in the stomach (pH 2) or the intestines (pH 7). (a) How would you set up an experiment to test this? (b) You hypothesize that the enzyme is more active at neutral pH. Draw a graph of the expected results (similar to Plot 5 of this lab) if your hypothesis is correct.

Solution

Amylase is a chemical that condensations sugars. It is made in the pancreas and the organs that make spit. At the point when the pancreas is sick or aroused, amylase discharges into the blood.A test should be possible to quantify the level of this protein in your blood.

Amylase might be measured with an amylase pee test.

Starch hydrolysis is performed for trial of amylase.