The function of a dichroic beam splitter in a fluorescence m

The function of a dichroic beam splitter in a fluorescence microscope can best be described as which of the following? Select only wavelengths that excite a molecule of interest allow visualization of only light coming from a fluorescent molecule of interest direct excitation light away from the observer, but allow light from the specimen to reach the observer create additional contrast in the final image of a cell

Solution

Answer is part 3: \"Dichoric beam splitters allow direct excitation light away from the observer but allow light from the specimen to reach the observer\"

Explanation: These splitters are also known as Dichromatic beamsplitters . They are specialized filters with dual function; They are designed in such a manner that they reflect excitation wavelengths (thus allow direct excitation light away from the observer) and pass emission wavelengths (allow light from the specimen to reach the observer). They are positioned in the light path right after the exciter filter and before the barrier filter. Therefore,  it allows some particular wavelength to pass through and allow the other wavelengths to be reflected.