you note an anomaly at your clinic in Beverly Hills around F

you note an anomaly at your clinic in Beverly Hills around Feb-14. A.Schwarzenegger buys roses reluctantly (Facultative-Romantic), R.Gosling buys them constantly (Constitutive - Romantic),& J.Nicholson refuses (non-Romantic). As a physicain with deep insights in cell& Mol Biol., you analyse blood samples-white blood cells (WBCs) on Jan-1 (NY) and Feb-14 for the protein Fabio & the gene Cupid.

1)Based on the structure of mouse mini-Fabio, do you predict that mice are predisposed to romantic behaviour or do they display the (human-like) + state & why? Amino acids noted in human-Fabio are conserved in mini-Fabio.

2) Could you make eternally un-romantic mice by a single mis-sense mutation in mini-Fabio?

what kind of information do you need?..I asked our professor about it but he told me all the information that you will need it to answer the questions are in the figure....!

Data sets are fictional Only for education entertainment. CS60xC N749-8760 Fabio 30 kDa 20 kDa 25 kDa 25 kDa mini-Fabio 20 kDa kDa 25 25 kDa Aahnold L_Ryan Gosling lack Nicholson NY NY Treat WBCs with: SDS+2Mercaptoethanol GelElectrophoresis Western Blots 100 DP 100 70 70 100 70 45 45 45 30 25 25 S=Soluble NY NY NY SPS P S PSP SP PSP P=Pellet 9% 0 100 10 90 % 90 1090 10 % 0 100 0 100 NY Northern Blot - Cupid GAPDH 100 kDa = *-aggregates 70 kDa = Monomer (y-NoMore) 45 kDa = Forms Dimer Edman Degradation 100 kDa = MEDRLFECHTER 70 kDa S MYVALENTINE 45 kDa MYVALENTINE - ATCTICAV

Solution

Answer 1)

100KDa forms aggregates. These aggregates are found in all the three humans; but the number of aggregates in Gosling is less in comparison to Aahnold and Nicholson. 45KDa dimer results in the protein \"MYVALENTINE\". This protein is the protein responsible for romantic behavior.

The 45Kda dimer is the Fabio gene protein itself; which is necessary for romantic behavior.

Mouse has mini-Fabio. This gene lacks a 30KDa fragment from the Fabio gene.

But still it exhibits 70KDa fragment which codes the protein \'MYVALENTINE\'. So, mouse is predisposed to romantic behavior.

Answer 2)

A single missense mutant is mutant 5. Please note that the non-romantic mice should have the protein \'IHATETHISDAY\'. For this 25KDa protein should accumulate; and this accumulation occurs in mutant 5, i.e. K620A.