2 You have been recently hired as a lab technician at a loca

2. You have been recently hired as a lab technician at a local research facility and you have been asked to prepare bacterial growth media supplemented with ampicillin. You have been asked for 200ml media containing 0.1mg/ml ampicillin. You have been given a stock solution of 100mg/ml. How much of the stock do you add to your media?

Solution

given that stock of the ampicillin is 100mg/ml

working concentration is 0.1mg/ml

amount of amphicilin added in 200 ml media will be

M1V1= M2V2

here M1 is the stock solution of ampicillin

V1 volume to be added to 200 ml media

M2 working concentration

V2 volume of media

volume added to 200ml is 0.2ml