Calculate the volume of your DNA stock and water you would n

Calculate the volume of your DNA stock and water you would need to prepare 100uL of a 50ng/uL solution.

Solution

Ans. To make calculation we must have the concentration of stock solution.

Concentration of DNA stock solution is not provided. So, to make the illustration more explanatory, I assume that the concentration of DNA stock solution = 500 ng/ µL

Use the formula-

            C1V1 = C2V2

C1= Concentration of initial solution 1, V1= volume of initial solution 1      ;( Stock solution)

C2= Concentration of final solution 2, V2= volume of final solution 2        ; [diluted solution]

We have,

            C1 = 500 ng/ µL          , V1 = ?

            C2 = 50 ng/ µL            ; V2 = 100 µL

Putting the values in above equation-

            500 ng/ µL x v1 = 50 ng/ µL x 100 µL

            Or, V1 = (50 ng/ µL x 100 µL) / 500 ng

            Hence, V1= 10 µL

Preparation of working solution: Add 10.0 µL of stock solution to 90.0 µL of distilled water. The total volume of the solution is 100.0 µL, the concentration = 50 ng/ µL.

Note: Please put the desired concentration of DNA stock solution while making calculation.