Please answer the following question completely and show all

Please answer the following question completely and show all work in order to be rated. Thanks!

What is the best way to determine if a biochemical reaction is entropically favorable/unfavorable and enthalpically faborable/unfaborable?

You purify your protease but are sad to disoovi d discover that is inactive. Yo ncubate the purified protei (bacter do you know? has regained function. What was wrong with your purified protease, and very briefly how Now that you can purity functional protease, you determine that the optimal pH for its activity is 6.4 a. Which of the listed buffers would you use and why b. You need to make this buffer at a concentration of 100 mM. Calculate the concentrations ofthe acidic and basic companents in your new buffer.

Solution

For a reaction to be enthalapically favorable the value of G-gibbs energy can be calculated experimentally. If the value of G-gibbs energy comes to be negative hence this reaction is spontaneous and favorable..