You did 5 fold dilutions and with the aid of an assay you wi

You did 5 fold dilutions and with the aid of an assay you will learn about it in a few weeks time, you were able to estimate that the concentration of antibody specific for the H1N1 virus (Influenza) in the 7^th dilution tube is 5 ng/1 ml

a). Calculate the concentration, in mg/ml, of anti H1N1 virus antibody present in the serum of an infected individual employing the “Dilution Factor”. (Consider the serum as your “stock”)

b). Calculate the concentration of the same antibody in tubes 6 and 8 of the same dilution series again employing the “Dilution Factor”

Solution

Answer:

Given: concentration of H1N1 virus in the 7th dilution tube is 5 ng/1 ml

Each tube is 5 fold dilutions

Dilution factor in 7th tube is = x x x x x x =1/78125

Concentration in original stock = 5 ng/mL x 78125 = 390625 ng/mL

a). Calculate the concentration, in mg/ml,

1000000 ng ---->1mg

390625 ng/mL =0.390625 mg/mL

(b) dilution factor in tube 6 is 15625 folds that of stock

Therefore, the concentration of the same antibody in tube 6 is = (Concentration in original stock)/15625

= 390625/15625

=25 mg/mL

dilution factor in tube 8 is 390625 folds that of stock

Therefore, the concentration of the same antibody in tube 8 is = (Concentration in original stock)/390625

= 390625/390625

=1 mg/mL