What would happen if the amount of tryptophan being produced
Solution
Ans. E. coli synthesizes tryptophan (an amino acid) through the expression of “trp operon”.
#1. When tryptophan is available in the surroundings (extracellular environment), the bacterium imports (uptakes) it. Thus, the cell can compensate its need for tryptophan without requiring trp operon expression.
#2. When there is scarcity of trp in the extracellular environment, E. coli needs to synthesize it to compensate its need. For that, it first need to transcribe “trp operon” genes – the mRNA of which are translated to produce enzymes that subsequently mediate tryptophan biosynthesis.
If RNA pol is destroyed, the cell can no longer transcribe trp operon into respective mRNAs. In absence of trp mRNAs, the cell can’t synthesize tryptophan. As a result, tryptophan concentration is depleted in the cell.
