Describe one other method for breaking open bacteria other t

Describe one other method for breaking open bacteria( other than lysozyme) How does it compare to lysozyme in terms of time, cost, efficiency, equipment needed?

Solution

Liquid based homogenization is the most commonly used technique for braking open bacteria.The French Press is the most commonly used homogenizer in this category. In this technique, high pressure is used to force cells through a narrow orifice,leading to cell lysis because of shear forces.This technique takes about 15-20 minutes to disrupt cells in a suspension.This method is expensive in terms of lyzozyme usage. The efficiency of French Press is over 90%. It can cause the lysis of about 90% in commonly cell types.The equipment consists of a hydraulin pump that is used to drive a piston. The piston forces the liquid sample through the tiny value at high pressure.The shear force experienced by the lquid during passage through the valve causes cell disruption.