answer below five MOLECULAR BIOLOGY question 4145 in your ow

answer below five MOLECULAR BIOLOGY question 41-45 in your own words. Do not copy paste answer from any direct sources otherwise it will reported as a spam. Use your own word upto 4-5 sentences. Credit will be gievn only if answer all five questions.

41. Which groove contains more nformation\" and why? How do proteins interact fically with this DNA groove. spec as when a zinc finger crosses the groove 42. Which of the following base pairs could proteins distinguish, and why? a. Major groove base pairs (GC/CG, AT/TA, GC/TA, GC/AT) b. Minor groove base pairs (GC/CG, AT/TA, GC/TA, GC/AT) 43. Where do you find A DNA, B DNA, Z DNA, and quadruplex DNA? 44. What contributes to DNA stability with regards to Tm? Which type of base pairs take the most energy to pull apart e. require a higher temperature to dissociate)? /Trm 45. Why would it take longer for more complex DNA to reanneal?

Solution

41:- The major grove of double helix DNA contains more information beacuse RNA polymerase transcribes most of the bases into RNA as it contains most of information for protein. The N7 and C6 atoms of Adenine and Guanine and the C4 and C5 atoms of cytosine and thymine face into the major groove, thus they can make interactions with amino acids in DNA-binding proteins.

43:-All forms of DNA are found in eukaryotic cell under different conditions B form is common under physiological pH. A forn under low humidity high salt concentration. Z is left handed and laboratory anamoly.

44:- GC content contributes to DNA stabiliwith regards to Tm. Guanine and Cytosine (GC) base pairs take most energy to pull apart duebto three hydrogen bonds than AT pair. This higher GC content more temperature is required to melt DNA.

45:-complex DNA after denaturation is not able to form hydrogen bonds against its base pair as it doea not orient properly after melting.