After performing four PCR experiments you run a gel to deter

After performing four PCR experiments, you run a gel to determine if your product was correctly made. You are looking for a band that is about 460 base pairs. Give an explanation as to the appearance of the 789bp band in the experiment for lane 2. Give one solution to correcting this problem.

Solution

Answer:

The 789bp band appears to be a nonspecific band. To reduce nonspecific products in PCR use fewer cycles when template concentration is high, and iincrease the number of cycles when template concentration is low. Optimize and shorten the annealing time. When the annealing temperature is too low, primers may bind nonspecifically to the template forming spurious products..