After collecting a clean catch urine sample how does one qua

After collecting a clean catch urine sample, how does one quantitatively test the sample? How is the sample prepared? How does one determine the number of microorganisms that are present in the urine sample? Be specific. Give an example.

Solution

The UTI (urinary tract infections) causing bacteria or fungal microbial species enter through the urethra (in men) or vulva (in women) via sexual activity or catheterization is the most common cause of UTI’s in women due to their anatomy (close distance to urethra). After invading the bladder, the E. coli attaches to the bladder walls and form a biofilm, which is resistant to the immune response of the body. These UTI\'s can be detected using clean catch urine sample collection or midstream clean -catch urine specimen collection

After collecting a clean catch urine sample, one can quantitatively test the sample in diagnosis microbiology laboratory and it is generally done by the streak plate technique. Sample is prepared by diluting the 1 ml of urine sample followed by culturing in suitable \"organic medium\" (ex. agar) and incubating for 24 to 48 hours to observed the growth of microbial species. Laboratory examination of urine and culture of urine samples is done may produce CFUs/mL greater than 10^3 then it is said to be positive.

Germ Tube Test is a screening diagnostic test after collecting a clean catch urine sample. The differential diagnosis of this patient is obtain some \"midstream urine samples\" for laboratory cultures in carbohydrate medium (measure adequate amount) as \"pathogenic organism can reproduce by budding\" means it is \"pathogenic fungi\" infected at the surgical region of cervix. Therefore, it will produce germ tube with fungal spores upon cell cultures (steak plate or pour plate method using agar as medium) & culture will produce \"yellow precipitate\" when examined with I2-KI & NaOH (iodoform test) due to alcohol formation as a metabolic byproduct. The morphology of diagnostic samples with infected pathogen can be observed under the microscope.

Urineanalysis can be performed quantitatively to check the levels of white blood cells, leukocyte esterase & urinary nitrites or bacteria under microscope as a result it is possible to detect any presence of metabolic disorders