MT3ECO CTC CCC ATG CTT ACA AGC AAG T MT4PST GGG TGA TAG ACC

MT3-ECO: CTC CCC ATG CTT ACA AGC AAG T MT4-PST: GGG TGA TAG ACC TGT GAT CCA TCG a) How many nucleotides are in each primer? b) Are these primers ssDNA or dsDNA? c) Which end of each primer is the 5’ end? The 3’ end?

Solution

Answer:

a. MT3-ECO: CTC CCC ATG CTT ACA AGC AAG T

The number of nucleotides in the primer is: 22

MT4-PST: GGG TGA TAG ACC TGT GAT CCA TCG

The number of nucleotides in the primer is: 24

b. The two primers are ssDNA.

c. MT3-ECO is the forward primer

MT4-PST is the reverse primer