Some labs run a sample of saline through the PCR and gel Wou

Some labs run a sample of saline through the PCR and gel. Would you expect to see a band in the lane for that sample? Why or why not? What would it mean if you do see a band in that lane? Did you have a band in Lane 5? If so, what does that indicate, and how does it affect your interpretation of your results

Solution

No, the sample of saline does not provide a band to see if it is pure. Because the saline sample is considered as negative control (does not contain the sample to be identified or measured). If the band appear with the saline sample means the sample is contaminated.