techniques is used to separate undigested chromosome please

techniques is used to separate undigested chromosome?

please choose the correct answer. thank you!

echniques is used to separate undigested chromosome? Select one: a. Fluorescent in situ hybridization b. Optical mapping c. Polyacrylamide gel electrophoresis X d. orthogonal field alternation gel electrophoresis

Solution

Ans.d

During normal gel electrophoresis method, large dna molecules of over 15-20kb moved in the zame direction irrespective of their sizes. So a suitable method was developed where in the voltage was alternated in three different directions.

Though this method takes longer duration then the conventional technique but is helpful to seperate undigested dna chromosomes.