design an experiment with a GFP green fluorescent orptein fu

design an experiment with a GFP (green fluorescent orptein) fusion protein to answer a specific research question. find an article that is studying a research area of interest using model organisms such as C. elegance . please INCLUDE CITATION for the article that your basing your experiment on. and how would you use dark or bright field microscopy to answer your question.

i need a qustion and a hypothesis.

Solution

Green fluorescent protein is a protein composed of 238 amino acid residue that emit green fluorescent when exposed to light in the blue to ultra violet range.It can be used in plasmid like pGLO plasmid is an engineered plasmid can be used as vector for creating new genetically modified organism.It can be used to tag protein in various studies of cells and tissues. It will fluorescence in which tagged gene is expressed. Fluorescent reporter protein is use to analyze live cell by fluorescence microscopy.

Bright field microscopy is used observing natural color of specimen or of stained sample . The object ( specimen) looks darker on a bright field microscope is used to view fixed or live specimen staining is required to cause contrast that allow them to be visible under microscope