1 5 TACGGTCGTTTACGCCTGCCCAATGCGTGC 3 a design primers that c

1)

5’ TACGGTCGTTTACGC------------------------------------CTGCCCAATGCGTGC 3’

a) design primers that can be used in a PCR reaction make each primer 15 nt

b) design a second set of primers that contains an EcoRI restriction site in each primer.

Solution

(a) :- First primer is CGTAACTACCCGTAT and the Second primer is TCATTTGGCAATACAT.

(b) :- First primer with EcoRI restriction site is CGTAAGAATTCCTACCCGTAT and second primer is TCATTTGAATTCGGCAATACAT.