You are given the stock solution for Carbenicillin but you c

You are given the stock solution for Carbenicillin, but you can\'t really read what the concentration of it is, so you have to look in the lab manual.

Now, you are tasked with preparing 27 ml of liquid media, that contains 50 MICROgrams/ml of carbenicillin.

Ho much Carbenicillin stock will you add to the 27 ml of media ? The answer MUST be in MICROLITERS, and type it just as a number with no units

(see calculations handout for help and practice; these are calculations form genetics that we are refreshing).

Solution

Use the formula:

N1 V1 (stock) = N2 V2 (working solution)

N1 V1 = 50 ppm * 27 ml

Please note that you need to know the concentration of stock solution of Carbenicillin for the same. Let the concentration be 100mg/ml

1 ppm = 1 microgm/ml = 1 mg/litre

So, 100mg/ml = 100 * 1000microgm/ml = 100000 ppm= This is the concentration of stock.

10^5 * V1 = 50*27

V1 = 1350 * 10⁵ = 0.01350 ml = 13.5 microliter

Add 13.5 microliter stock to 26986.5 microliter of media. This will make 27ml media containing 50microgm/ml of carbenicillin.

Note: Please change the values accordingly if stock concentration differs.