Which of the following changes will result in an increase in

Which of the following changes will result in an increase in the resolution of an infinity-corrected microscope? Select all that are true. Using an objective lens with a longer focal length. Using an objective lens with a larger diameter. Using light with a longer wavelength. Using an immersion liquid with an index of refraction greater than the index of refraction of air.

Solution

correct: a, d

the shorter the wavelength, the higher the resolution. So not option C.

the refractive index increases the speed of the light passing through a medium is slower. As light slows down the wavelength gets shorter and yields better resolution.

The numerical aperture of a microscope objective is a measure of its ability to gather light and resolve fine specimen detail at a fixed object distance. Image-forming light waves pass through the specimen and enter the objective in an inverted cone.

aperture = f/D so f should be more and D should be less. f= focal length, D = diameter.