Below is a restriction map of a plasmid Match the lane up wi

Below is a restriction map of a plasmid. Match the lane up with the enzymes used to digest the intact, circular plasmid.

Solution

Lane 1: Single digestion with either HindIII or SacI.
Lane 2: EcoRI and HindIII digestion would give restriction fragments of 250, 3250 and 3500 units.
Lane3: EcoRI digestion would give two restriction fragments of 3500 units each.
Lane4: EcoRI and SacI digestion would give three fragments of 3500, 2000 and 1500 units.