Arrange the following enzymes in the order of their action d

Arrange the following enzymes in the order of their action during DNA replication in E. coli: DNA polymerase 1, DNA polymerase 3, DNA primate, DNA gyrate and DNA helicase.

Solution

In E.coli, the replication starts at a single origin site (Monocistronic), it is semi-conservative and consists of two replication forks i.e. leading strand (Continuous DNA replication) and lagging strand (Okazaki fragments). The replication proceeds sequentially as below:

Therefore, the order of events during DNA replication in E.coli are:

DNA helicase, DNA primate, DNA polymerase 3, DNA polymerase 1, and DNA gyrate