A stock solution of a 1012 cellsml is serially diluted 12 by
A stock solution of a 10^12 cells/ml is serially diluted 1:2 by combining 1ml of the stock solution with 1ml of PBS. Complete the diagram below by inserting the resulting concentration of cells in each tube in the blanks provided and the total dilution factor in the space at the bottom of each tube. Put Concentration cells/ml for each tube into each of the blanks at the top of the tubes and fill in the total dilution factor in each of the blanks at the bottom.
Solution
Let the total volume of solutions in each test tube is 10 ml..!!
So we take 1ml of stock solutions and add to the each tube in 9 ml of water the dilution factors will be..
First tube 10^-1, cells - 10^11 cells/ml
Second tube - 10^-2, cells - 10^10 cells/ml
Third tube - 10^-3,, cells-10^9 cells/ml
Fourth tube - 10^-4,, cells-10^8 xells/ml
Fifth tube - 10^-5,, cells-10^7 cells/ml
Sixth tube- 10^-6,, cells-10^8 cells/ml
Seventh tube - 10^-7,, cells-10^7 cells/ml
