Should the enzyme lactase be able to cleave sucrose Why The

Should the enzyme lactase be able to cleave sucrose? Why? The wavelength of yellow light is about 580 nm. If 0-nitrophenol is yellow, why do you set the spectrophotometer to 410 am? Consider the free energy equation: Delta G = Delta H-T Delta S: The reaction catalyzed by lactase has a negative Delta G and therefore proceeds with the aid of the enzyme. Does this reaction have a negative or positive Delta S? Explain If you did this reaction with lactose as the substrate, rather than ONPG, what absorbance result would you expect to see? Why? Draw the equation for the reaction happening when you add concentrated NaOH in #5. What is happening to the H^+ concentration of the solution?

Solution

1. the enzyme lactase could break down sucrose as it is similar in structure to lactose but the enzyme in reality only breaks lactose because of the shape of lactose. the physical interaction is not possible between lactase and sucrose.