A new species of sea horse has been discovered and is being

A new species of sea horse has been discovered and is being sequenced. The genome of the new species is being aligned using the reference sequence from a already-known species of sea horse. Since the two species are evolutionarily diverged, there may be some differences between their sequences. a) Align the set of mini-fragments above. b) How are the sequences of the two sea horse species different? c) Would this alignment have been easier or more difficult if you didn\'t have any reference sequence at all (alignment without a reference is called \"de novo\")? Explain. d) If there had been sequencing errors, would this alignment have been easier or more difficult? Explain. e) What is the depth of coverage?

Solution

5\'------------ACCTACATTCACAGAATGAT----------3\'..............reference sequence 3\'------------TGGATGTAAGTGTCTTACTA-----------5\'.............is the complementary sequence ACCTAC.....ATTCACA.....G......AATGAT ACCTAC.........ACCTAG has been observed....a pyrimidine replaced another pyrimidine ATTCACA..............ATTCACT has been observed....a purine replaced another purine G.... has been deleted AATGAT ACCTACATTCACAGAATGAT...older sea horse the order has changed to ACCTAGATTCACTAATGAT...new species Due to deletions, insertions and duplication at gene level, leads to changes in the traits of the new species of sea horse.