For the following guided essay on prokaryotic translation fi

For the following guided essay on prokaryotic translation, fill in the appropriate areas. If the actor of the step is given on the arrow, you will provide in the box the process performed by that actor. If the process is given in the box, you must label the arrow with the actor involved.

Solution

Prokaryotic Translation

1. The tRNA gets charged with N-formylmethionine. 30S subunit binds to the mRNA template at purine rich region upstream AUG codon.

2. It helps in the assembly of the initiation complex. The A site is the point of entry for aminoacyl tRNA while the P site is where peptidyl tRNA is formed in the ribosome.

3. Elongation starts when the fMet-tRNA enters the P site, causing a conformational change which opens the A site for the new aminoacyl-tRNA to bind. This binding is facilitated by elongation factor-Tu (EF-Tu), a small GTPase.

4. Termination Codons.

5. These factors trigger the hydrolysis of the ester bond in peptidyl-tRNA and the release of the newly synthesized protein from the ribosome. A third release factor RF-3 catalyzes the release of RF-1 and RF-2 at the end of the termination process.