We would like to purify in the active form protein containin

We would like to purify in the active form protein containing pI = 6.5 by ion-exchange chromatography. Protein loses activity in acidic pH. Which resin can we use for purification of this protein, and why? Cation-exchange Anion-exchange Explain:

Solution

Answer: An-ion excahnge chromatography can be used for the purification of the protein with out looosing the value.

examples for the anion excahnger are as follows

Generally the resins are cross linked Which resin is used to purify the protein of pI of 6.5 by ion exchange polymers of polystyrene. Cross linking decreases the ion capacity of resin and prolongs time needed to accomplish the ion exchange process but improves the robustness of the resin. Particle size also influences the resin parameters, smaller particles have outer surface but causes larger head loss in the column process.

Four main types of ion-exchange resins differ in their functional groups: