The following is the DNA sequence of Gene Q that you want to

The following is the DNA sequence of Gene Q that you want to amplify using the polymerase chain reaction (PCA) technique. Name all the components that you would need to carry out the PCR reaction. Briefly state the function of each of these components. Design born forward and reverse primers that can be used to amplify Gene Q. Each primer should be 15bp in length Compare and contrast the process of PCR amplification and cellular DNA replication.

Solution

The given gene sequence is :

5\'-CTCGAGGTGAATATGAAAGCATTTGGCGCGTAATCGATA-3\'

3\'-GAGCTCCACTTATACTTTCGTAAACCGCGCATTAGCTAT-5\'

which needs to be amplified.