PCR requires which of the following check all that apply tra

PCR requires which of the following (check all that apply)? transcription unwinding DNA using helicase transformation Unwinding DNA using heat RNA Pol translation nucleotides amino acids tRNA nuclear pore thermal cycler Taq Pol For all of the responses you selected in question # 1, indicate why they are required for PCR. For all those you did NOT select they are required for PCR.

Solution

PCR requires

transcription ( for amplifying DNA from RNA)

Unwinding DNA using helicase (Helicase-dependent amplification)

Unwinding DNA using temperature (Annealing temperature, Cooling temperature)

RNA Pol: during transcription using DNA as templates

Nucleotides (Miniprimer PCR)

tRNA (genotyping Entamoeba histolytica)

Nuclear Pore ( Q-PCR)

Thermal Cycler (Regulation of temperature)

Taq Pol (DNA Taq Pol:heat-stable DNA polymerase)

2.

Not required

Amino acids: Required for protein synthesis

Translocation: These are done to check the PCR.

Transformation: single-base overhang ligation is better than Transformation