For this problem consider the following sequence which is th

For this problem, consider the following sequence, which is the transcribed region of a short prokaryotic gene (only one strand is shown). The transcript is translated to produce a short polypeptide. The DNA sequence is formatted with a space every 5 nucleotides solely in order to make it easier to read. 5\' GTCTC CAAGG CTTAC CGTCA CCAAG GTCTC CGTGC GTACA TTAGG CCTGC TGCTG 3\' What is the sequence of the translation product? (label the ammo terminus)? What is the sequence of the transcription product? (label the 5\' terminus) What is the sequence of the coding strand of the transcribed part of this gene? (label the 5\' terminus) What is the sequence of the non-coding strand of the transcribed part of this gene? (label the 5\' terminus)

Solution

DNA strand:   5\' - GTCTC CAAGG CTTAC CGTCA CCAAG GTCTC CGTGC GTACA TTAGG CCTGC TGCTG - 3\'

mRNA strand: 3\' - CAG AGG UUC CGA AUG GCA GUG GUU CCA GAG GCA CGC AUG UAA UCC GGA CGA CGA C - 5\'

Amino acid sequence or translational product : Gln - Arg - Phe - Arg - Met - Ala - Val - Val - Pro - Glu - Ala - Arg - Met - STOP - Ser - Gly - Arg - Arg

Coding strand: 5\' - GTCTC CAAGG CTTAC CGTCA CCAAG GTCTC CGTGC GTACA TTAGG CCTGC TGCTG - 3\'

Noncoding strand: 3\' - CAGAG CTTCC GAATG GCAGT GGTTC CAGAG GCACG CATGT AATCC GGACG ACGAC - 5\'