Question 2 The following DNA sequence has been used as templ

Question 2: The following DNA sequence has been used as template for transcription, originating the mRNA that was translated to produce the corresponding polypeptide as follows.

DNA:   3’ A T A C T A G A C C T T C C T A A T C A A A 5’

mRNA: 5’ U A U G A U CU G G A A G G A U U A G U U U 3’

polypeptide: N – MET – ILE – TRP - LYS – ASP - C

Point mutations have been made to the original DNA template. In each case, write the mRNA and protein made from the new DNA molecule, identify the type of point mutation with respect to the change in the sequence of bases in the DNA template and the polypeptide translated (use ALL necessary terms to describe the mutation type).

b)      DNA:        3’ A T A C T A G A C T T T C C T A A T C A A A 5’

What type of point mutation was made in the DNA template above?

Will that point mutation affect the polypeptide translated from the mRNA transcribed from the DNA template? Explain

Solution

N – MET – ILE – TRP - LYS – ASP - C

the point mutation A-T conversion make mRNA seq

5’ U A U G A U CU G G U A G G A U U A G U U U 3’

where A convert to U make the translation

N – MET – ILE – TRP - Stop

so this mutation introduced one stop codon in between resulting into truncated protein and this kind of mutation known as misence mutation

M I W K D

M I W stop