Homework Sourse1 Which of the following statement is true for both DNA repl
1. Which of the following statement is true for both DNA replication and transcription?
Both polymerases catalyze the formation of phosphodiester bonds between nucleotides.
Primers are required to provide 3\'-OH group for both polymerases to initiate the synthesis of DNA or RNA.
DNA and RNA synthesis both proceed in a 5\' to 3\' direction or, in other words, nucleotides are added to the 3\'-OH groups of the growing chains.
Two of the above.
All of the above.
2. Consensus sequences that are involved in prokaryotic/bacterial transcription include...
the -10 consensus sequence or Pribnow box.
the -35 consensus sequence.
enahancer sequences.
Two of the above.
All of the above.
| A. | Both polymerases catalyze the formation of phosphodiester bonds between nucleotides. | |
| B. | Primers are required to provide 3\'-OH group for both polymerases to initiate the synthesis of DNA or RNA. | |
| C. | DNA and RNA synthesis both proceed in a 5\' to 3\' direction or, in other words, nucleotides are added to the 3\'-OH groups of the growing chains. | |
| D. | Two of the above. | |
| E. | All of the above. |
Solution
1. A & C are correct answers. So option D is correct. Between nucleotides Phosphodiester bond exist. So during polymerization, both DNA and RNA polymerases add one by one nucleotides by forming Phospho di ester bond. DNA replication require primer but not transcription not need. All polymerases in in vivo conditions, synthecize in 5\' - 3\' direction
2. Answer is E. The elements represented in A, B and C are Upstream control elements like promoters and enhancers. All are cis elements and all promotes gene expression. They are most commonly present in upstream side of the gene
