Design an experiment using RNA polymerase and a single DNA t

Design an experiment using RNA polymerase and a single DNA target encoding a protein of your choosing that will allow you to screen for random protein-protein interactions. Note how you would select for successful interactions and include a clear set of controls.

Solution

cDNA cloning is an experiment using \"RNA polymerase and a single DNA target encoding a protein\" of your choosing that will allow you to screen for random protein-protein interaction finally compared to the control groups. For example, the experiments that showed initiators and promoters & RNA polymerase (staining done using immunohistochemistry & flow cytometry) can work together to promote papilloma and sustained papilloma formation includes selection healthy transgenic mice and followed by chemically induced skin carcinogenesis through “initiators” & “promoters” & binding of RNA polymerase and a single DNA target encoding a protein in papilloma.

The initiator agents such as DMBA or initiator benzopyrane used to alter “genes Hras1 & Kras (a single DNA target encoding a protein)” used as physical gents to induce mutations in the keratinocytes of transgenic mice. Some times N-methyl-N’-nitro-N-nitrosoguanidine or UV light is used to induce mutations in the above genes so that RNA polymerase cannot bound to it finally \"papilloma protein may developed\" when compared to control groups. These protocols are initiates lesions of pipilloma in the skin & exposed to promoters such as “telocidin”, “okadaic acid” alters protein kinase C or protein phosphatases finally generates oxidative stress to generate tumors of sustained papilloma (epidermal hyperplasia due to higher epidermal layers). These events can be observed after isolating skin samples subjected to “immunohistochemistry with specific antibodies” followed by cDNA analysis and microarray analysis to unravel A T transversion occurred in codon 61 of the Hras1 gene