6 Design a positive selection for each of the following type

6. Design a positive selection for each of the following types of mutants. Discuss what kind of selective plates and/or conditions you would use?

a. Mutants resistant to the antibiotic coumermycin

b. Revertants of a trp mutation that makes cells require tryptophan.

Solution

Mutants resistant to antibiotic coumermycin;

Revertants of a trp mutation that makes cells require tryptophan.