Design a DNA sequence to introduce 4 copies of tandem HA tag

Design a DNA sequence to introduce 4 copies of tandem HA tags on N-terminus of the following hypothetical protein. How will you apply this tag to detect your protein in a mix? N Protein of interest C

Solution

DNA SEQUENCE TO INTRODUCE 4 COPIES OF TANDEM HA TAGS ON N TERMINUS:

TAC CCA TAC GAT GTT CCA GAT TAC GCT TAC CCA TAC GAT GTT CCA GAT TAC GCT TAC CCA TAC GAT GTT CCA GAT TAC GCT TAC CCA TAC GAT GTT CCA GAT TAC GCT

DETECTION OF PROTEIN USING THIS TAG