12 We have done a nutrient recovery experiment for Hec and T

12.

We have done a nutrient recovery experiment for Hec and Trp. Below are the growth results of plating nine colonies on plates containing different media compositions. Indicate which colonies have a loss of function mutation for the given genotypes. When filling in the blank, use the colony numbers in numerical order separated by commas only. No spaces. For example in the box enter: 1, 2, 6, 10

Solution

Mutant Genotype Colony(ies) Hec- 4 Trp- 1,5 Hec- & Trp- 3,9 In medium when only hec is applied colony-4 is expressed, so without hec this colony shows loss of function mutation. In medium when only trp is applied colony-1,5 is expressed, means when trp is absent these colonies show loss of function mutation. In medium when hec & trp bothis applied colony-3,9 expressed along with other colonies, so when hec and trp both are absent in the medium these two colonies show loss of function mutation.