Describe where your amylase gene is at each step of CySI wh

Describe \"where\" your amylase gene is at each step of CySI & what is happening at each step. (For example: the amylase gene is in the vector)

Solution

Step

Where is the amylase gene

What is happening during this step

Genomic isolation

In Genome

The DNA genomic DNA extraction will helps in further analysis

PCR

PCR vial

The amylase gene will be amplified

Purification

PCR vial

To remove all other unwanted materials except amylase amplicon

Ligation and clonig

Plasmid

Facilitates for transformation

Tranfomration

Host

Helps in expression and maintaininence

Screening and selection

Host

Helps in the idenfication of transformed host

Step	Where is the amylase gene	What is happening during this step
Genomic isolation	In Genome	The DNA genomic DNA extraction will helps in further analysis
PCR	PCR vial	The amylase gene will be amplified
Purification	PCR vial	To remove all other unwanted materials except amylase amplicon
Ligation and clonig	Plasmid	Facilitates for transformation
Tranfomration	Host	Helps in expression and maintaininence
Screening and selection	Host	Helps in the idenfication of transformed host